Photoaffinity labelling of a nitrobenzylthioinosine-binding polypeptide from cultured Novikoff hepatoma cells
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منابع مشابه
Photoaffinity labelling of a nitrobenzylthioinosine-binding polypeptide from cultured Novikoff hepatoma cells.
Site-specific binding of nitrobenzylthioinosine (NBMPR) to plasma membranes of some animal cells results in the inhibition of the facilitated diffusion of nucleosides. The present study showed that nucleoside transport in Novikoff UA rat hepatoma cells is insensitive to site-saturating concentrations of NBMPR. Equilibrium binding experiments demonstrated the presence of high-affinity sites for ...
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A study of the enzymes of the glycogen pathway in Novikoff ascites hepatoma shows that glycogen synthetase has the lowest activity and that the tumour contains no high-K(m) soluble glucokinase. However, incubation of tumour cells with metabolizable sugars in vitro, or intraperitoneal administration of glucose into the tumour-bearing rat, results in glycogen accumulation by the tumour cells. Gly...
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Inhibition of fermentation or of glucose uptake in the presence ofoxygen has been observed in many forms of life and is generally referred to as the 'Pasteur effect'. In most tissues, oxidative inhibition of fermentation (ethanol or lactate production) is reflected by a parallel decrease in glucose consumption. Since certain tissues utilize lactate significantly, estimation of the Pasteur effec...
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As estimated from initial uptake rates, the uptake of glucosamine-l-14C by cultured Novikoff rat hepatoma cells in glucose-free basal medium follows normal Michaelis-Menten kinetics, with an apparent Km of about 20 mM. Since glucosamine is not accumulated by the cells against a concentration gradient, the main mode of uptake is by facilitated diffusion, but other evidence indicates that glucosa...
متن کاملPurification of a ribonuclease inhibitor from Novikoff hepatoma supernatant fraction.
A comparison between the levels of ribonuclease inhibitor in normal rat liver and Novikoff hepatoma 105,000 X g supernatant fractions is reported. Novikoff hepatoma con tained 61% less inhibitor per g of tissue or 39% less per mg protein than did normal rat liver. A rapid and simple method that had been developed for the purification of rat liver ribonuclease inhibitor was applied to Novikoff h...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1986
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2360665